CTAB Protocol for the Isolation of DNA from Plant TissuesTE Buffer 10 mM Tris pH 8 1 mM EDTA Method Plant samples can be prepared by cryogenically grinding tissue in a mortar and pestle after chilling in liquid nitrogen Freeze dried plants can be ground at room temperature In either case a fine powder is best for extracting DNA For each 100 mg homogenized tissue use 500 µl of CTAB